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TOOLS FOR RBTI TESTING OF SALIVA

 
TOOLS FOR RBTI TESTING OF SALIVA AND FRESHLY VOIDED URINE
FIRST: study mass of urine with light to estimate cell debris...RECORD CELL DEBRIS
Put one drop of saliva in well depressions D & E...
Put four drops of ammonia nitrogen reagent in well depression F...
Put six drops Universal Extracting Solution (UES) in test tube or nurse's pill dispenser cup...
Put one drop of urine in well depression A & B; one drop of urine in test tube (cup) and two drops of urine on refractometer prism...
Close cover plate on refractometer and read scale...RECORD BRIX
Agitate mixture in test tube (cup) for 1 minute or 120 squeezes...
Put one drop of agitated mixture in well depressions C and F...
Wait 15 seconds and compare well depression F to ammonia nitrogen card...RECORD AMMONIA NUMBER
Drop four drops of nitrate nitrogen reagent into well depression C at 4 points of compass...
Put one drop of Bromthymol Blue pH reagent in well depressions A & D...
Observe the pH colors forming in well depressions A & D. If either is on the acid side (below 6.40, yellowish) add one drop of Chlorphenol Red pH reagent to well depression next to it (B, E, or both if needed)...
If pH colors in either A or D or both are on the alkaline side (above 6.40---bluish) add one drop of Phenol Red pH reagent to the well depression next to it (B, E or both if necessary)...
NOTE: if after adding Chlorphenol Red pH reagent to B and/or E, and it appears that the urine or saliva is very acid (less than 5.40), use locations G and/or H to add either one drop of urine and/or saliva and then one drop of Bromcresol Green pH reagent...
Read urine and saliva pH using appropriate card matched to reagent...RECORD SALIVA pH AND URINE pH
Use conductivity (EC) meter to obtain µsiemens conductivity reading of urine and divide by 700...RECORD SALTS NUMBER
Compare well depression C with nitrate nitrogen card after 4-6 minutes...RECORD NITRATE NITROGEN NUMBER
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